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MUT'n'CUT (beta testing)

Identifies Unique Restriction Enzyme Sites Generated by Single Nucleotide Mutation.

The purpose of MUT'n'CUT is to define the location in a user-defined sequence of nucleotide changes which would generate unique restriction enzyme recognition sites. Furthermore, the program determines the effects the nucleotide mutation would have were it to occur within an open reading frame (ORF). This permits the researcher to introduce convenient cut sites while minimizing changes to the gene product; thereby minimizing deleterious effects in the organism.

This version of MUT'n'CUT does not consider mutations resulting from changes to more than one nucleotide.

Sequence contains:

NO introns
ONE or MORE introns

This program is presented here for beta testing

created: 22/10/00
last modified 8/11/00
Feedback is always appreciated: