Curriculum Vitae - Bruce Alan Williams

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Index

I) Educational Qualifications II) Working Experience III) Scholarships, Awards, Honours IV) Publications V) Technical Abilities VI) References
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I) Educational Qualifications

PhD 1984-1993. McGill University, Biology department. 1205 Dr. Penfield Ave., Montreal, Quebec. Title of thesis: Isolation and characterization of abscisic acid-responsive, embryo specific genes from Zea mays. Thesis supervisor: Dr. Adrian Tsang. B.Sc. 1978-1984. Combined honours: Chemistry and Biology. York University, 4700 Keele St., Downsview, Ontario. Title of undergraduate thesis: Basidiogenesis in Hericium coralloides: An ultrastructural study. Thesis supervisor: Dr. I. Brent Heath.
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II) Working Experience

Post-Doctoral Research: 1997-1998. Concordia University, Biology department. Nature of research: Saccharomyces cerevisiae genome project. Supervisor: Dr. Reginald Storms Post-Doctoral Research: 1994-1997. Concordia University, Biology department. Nature of research: Molecular study and genetic manipulation of Aspergillus niger. Supervisors: Drs. Etta Kaeffer, Reginald Storms and Adrian Tsang. Responsible for oligonucleotide synthesis, 1989. McGill University, Biology department. Direct supervisor: Kathryn Peterson Teaching assistant, 1984-1990. Biology 177-301; Laboratory in Molecular and Cellular Biology. McGill University, Biology department. Course Directors: Dr. R. Sinclair, Dr. R. Poole. Research assistant, summers of 1982, 1983, 1984. York University, Chemistry Department. Employer: Dr. G.O. Aspinall.
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III) Scholarships

FCAR Post-Graduate Fellowship (Quebec), 1987-1989. NSERC Post-Graduate Fellowship, 1984-1987. NSERC Undergraduate Summer Scholarship, 1982-1984.

Awards

Travel award to present results at the U.C.L.A. Symposium on The Molecular Basis of Plant Development. Steamboat Springs, Colorado. 1988. Sponsor: McGill University, Department of Biology. Best Undergraduate Thesis Award, 1984. Sponsor: Graduate Students Society, York University. Bertrand Gerstein Award, 1981. Sponsor: Bertrand Gerstein.

Honours

Chemical Society of Canada Award, 1983. Dean's Honour Roll, York University, 1982.
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IV) Publications

Refereed Journal Articles Published Ushinski SC, Bussey H, Ahmed AA, Wang Y, Friesen J, Williams BA, Storms RK, 1997. Histone H1 in Saccharomyces cerevisiae. Yeast 13:151-161. Williams BA, Sillaots S, Tsang A, Storms R, 1996. Isolation by genetic complementation of two differentially expressed genes for -isopropylmalate dehydrogenase from Aspergillus niger. Curr Genet 30:305-311. Williams BA, Tsang A, 1994. Analysis of multiple classes of abscisic acid-responsive genes during embryogenesis in Zea mays. Dev Gen 15:415-424. Williams BA, Tsang A, 1992. Nucleotide sequence of an abscisic acid-responsive, embryo-specific maize gene. Plant Physiol 100:1067-1068 Williams BA, Tsang A, 1991. A maize gene expressed during embryogenesis is abscisic acid-inducible and highly conserved. Plant Mol Biol 16: 919-923. Aspinall GO, Khondo L, Williams BA, 1987. The hex-5-enose degradation: cleavage of glycosiduronic acid linkages in modified methylated Sterculia gums. Can J Chem 65: 2069-2076. (c) Conference Abstracts Williams B, Tsang A, 1988. Abscisic acid is required to maintain normal levels of embryo-specific mRNAs in Zea mays. J Cell Biochem Supp 12C: 168.
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V) Technical Abilities

During post-doctoral research at Concordia University I have: - created an A. niger cDNA library in a yeast expression library. - sequenced more than 100 genes from this library. - analyzed codon usage in A. niger. - disrupted the histone H1 gene in S. cerevisiae. - created a gene fusion between S. cerevisiae histone H1 and the green fluorescence protein from Aequorea victoria and used fluorescence microscopy to demonstrate nuclear localization. - wrote several computer programs in Basic for the following operations: - to calculate codon bias indices from a database of nucleic acid sequences. - to calculate solution concentration from optical density. - to estimate fragment size from gel mobility. - to quantify similarity between related sequences. - to search for potential upstream activation sequences in promoter regions of genes. - to identify the most likely open reading frames within a genomic DNA sequence. - created a lab web site (www://clone.concordia.ca/aspergillus) for the dissemination of protocols, research summary, and permit access to novel programs for molecular biologists. In the course of my research at McGill I have: - created a maize cDNA library and screened it for differentially- expressed genes. - purified RNA and DNA from plants, bacteria, and bacteriophage. - subcloned DNA fragments. - sequenced single-strand and double-strand DNA using the dideoxy method. - transformed E.coli with DNA using Hanahan's method and via electroporation. - performed Northern and Southern blot analysis. - generated antibodies from rabbit to a fusion protein isolated from E.coli. - analysed proteins on 1-D and 2-D polyacrylamide gels. - performed Western blot analysis. - acquired a good working knowledge of MS-DOS, WordPerfect, DBase-III, PCGene, and various other software. As an undergraduate student at York I have: - prepared fungal tissue for and carried out examinations using visible light microscopy and transmission electron microscopy. - purified and chemically modified polysaccharides. - used HPLC to isolate chemical degradation products of modified polysaccharides. - used GLC, GLC-MS, NMR, and various spectrophotometers to identify reaction products.

VI) References

Prof. Adrian Tsang, Concordia University, Biology department. Phone # (514) 848-3402. Prof.Reginald Storms, Concordia University, Biology department. Phone # (514) 848-3412 Prof. Greg Brown, McGill University, Biology department. Phone # (514) 398-6426. Prof. Pat Gulick, Concordia University, Biology Department. Phone # (514) 848-3407.
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